dy7925-05  (r&d systems)

Journal: Molecular Medicine

Article Title: Panx3 deficiency in mice impairs bone fracture healing and causes transient hypoglycemia in neonatal animals

doi: 10.1186/s10020-026-01458-9

Figure Lengend Snippet: Panx3 deficiency results in altered glucose metabolism markers. A Serum levels of glucose and insulin in mice of the indicated genotypes at 3, 7 and 14 postoperative days ( N = 6). Relative fold changes to controls were plotted. B Heatmap of 30 differentially regulated genes with highest fold changes in the callus of Panx3 +/+ ( N = 5) and Panx3 −/− ( N = 4) mice 3 days after the osteotomy. Each column represents one individual sample and gene, respectively. Z-scores were calculated based on the expression level of each gene and visualized in a color scale (blue = downregulated, red = upregulated). Genes associated with glucose metabolism were highlighted in red. C Expression of the indicated genes ( Slc = solute carrier family, C1qtnf3 = C1q tumor necrosis factor-related protein 3) in the callus of Panx3 +/+ and Panx3 −/− mice at the indicated postoperatively time points ( N = 6). D Representative alizarin red–stained images of isolated calvarial osteoblasts from Panx3 + / + and Panx3 −/− mice and quantification of extracellular matrix mineralization after 10 days of osteogenic differentiation ( N = 6). E Expression of the indicated genes ( Bglap = bone gamma-carboxylglutamic acid-containing protein, osteocalcin, Dmp1 = dentin matrix acidic matrix phosphoprotein 1, Panx3 = pannexin 3, C1qtnf3 = C1q tumor necrosis factor-related protein 3, Slc = solute carrier) in calvarial osteoblasts from Panx3 + / + and Panx3 −/− mice after 10 days of osteogenic differentiation ( N = 4). Unpaired Student’s t test was used to determine statistical differences between groups at each time point or for each gene. Data are presented as box plots that represent median with minimum and maximum whiskers

Article Snippet: C1qTNF3 duo set ELISA (#DY7925-05, R&D Systems Inc., Minneapolis, USA) was used to determine the concentration in human patients.

Techniques: Expressing, Staining, Isolation

Journal: Molecular Medicine

Article Title: Panx3 deficiency in mice impairs bone fracture healing and causes transient hypoglycemia in neonatal animals

doi: 10.1186/s10020-026-01458-9

Figure Lengend Snippet: Panx3 -deficient mice display impaired glucose homeostasis. A Body weight of Panx3 + / + ( N = 4–6) and Panx3 −/− ( N = 3–4) mice and B serum glucose of Panx3 + / + ( N = 6) and Panx3 −/− ( N = 3–5) mice at the indicated ages. C Glucose and D insulin tolerance test of adult mice of the indicated genotypes ( N = 7). Relative alterations to the baseline level were plotted. E Serum levels of insulin and C1qtnf3 in Panx3 + / + and Panx3 −/− mice at the indicated ages ( N = 3 for P3, N = 7 for P600). F Serum concentration of C1qtnf3 in healthy individuals as control ( N = 10), patients displayed adequate fracture healing (Fx, N = 16) and nonunion formation (NU, N = 19). Unpaired Student’s t test was used to determine statistical differences between groups at each time point. Data are presented as box plots that represent median with minimum and maximum whiskers

Article Snippet: C1qTNF3 duo set ELISA (#DY7925-05, R&D Systems Inc., Minneapolis, USA) was used to determine the concentration in human patients.

Techniques: Concentration Assay, Control

Journal: Heliyon

Article Title: Obesity alters adipose tissue response to fasting and refeeding in women: A study on lipolytic and endocrine dynamics and acute insulin resistance

doi: 10.1016/j.heliyon.2024.e37875

Figure Lengend Snippet: Key resources table

Article Snippet: Human CTRP3 ELISA DuoSet Kit , R&D systems Biotechne , Cat# DY7925-05.

Techniques: Clinical Proteomics, Recombinant, Enzyme-linked Immunosorbent Assay, Software

Journal: PLoS ONE

Article Title: CTRP3 and serum triglycerides in children aged 7-10 years

doi: 10.1371/journal.pone.0241813

Figure Lengend Snippet: The mean, standard deviation, min and max values are reported for all serum values (n = 62).

Article Snippet: Total (unseparated samples), high molecular weight isomer (HMW), and middle molecular weight isomer (MMW) of CTRP3 were measured using the CTRP3 ELISA (R&D systems, Catalog number #DY7925-05).

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: CTRP3 and serum triglycerides in children aged 7-10 years

doi: 10.1371/journal.pone.0241813

Figure Lengend Snippet: The Spearman’s rank-order correlation coefficient was calculated between MMW CTRP3 and serum analytes. Each dot represents a unique individual. When a significant correlation exists the linear relationship is graphed with the solid line and the 95% confidence interval with the dotted lines. Abbreviations: n.s. = not significant, MMW CTRP3 = medium molecular weight CTRP3 oligomer (<300 kDa).

Article Snippet: Total (unseparated samples), high molecular weight isomer (HMW), and middle molecular weight isomer (MMW) of CTRP3 were measured using the CTRP3 ELISA (R&D systems, Catalog number #DY7925-05).

Techniques: Molecular Weight